Potential Prognostic Molecular Signatures in a Preclinical Model of Melanoma

Anticancer Res. 2017 Jan;37(1):143-148. doi: 10.21873/anticanres.11299.


Numerous studies have revealed a variety of pathways involved in the development of melanoma, however, the molecular and genetic divergence of underlying mechanisms remain vague. In a mouse model, we studied the expression pattern of insulin-like growth factor 2 mRNA-binding protein 1 (Igf2bp1) and target genes microphthalmia-associated transcription factor (Mitf), v-myc avian myelocytomatosis viral oncogene homolog (Myc), B-cell lymphoma 2 (Bcl2), prothymosin alpha (Ptma) and melan-A (Mlana) in relation to tumor-growth characteristics. The in vivo expression of the aforementioned genes was assessed by quantitative Real Time-Polymerase Chain Reaction (RT-PCR) in tumors established by B16-F1-derived clones. Gene expression was correlated with tumor growth characteristics. Simultaneous expression of elevated levels of Myc, Igf2bp1, Ptma and Mitf characterizes tumors with a more aggressive phenotype. Our findings introduce a tumor-specific molecular signature possibly associated with melanoma heterogeneity. The concomitant overexpression of key molecules such as IGF2BP1, PTMA, MYC and MITF could serve as prognostic or predictive marker.

Keywords: Igf2bp1; Melanoma; Ptma; molecular signatures; β-catenin/Wnt pathway.

MeSH terms

  • Animals
  • Biomarkers, Tumor / genetics*
  • Biomarkers, Tumor / metabolism
  • Cell Line, Tumor
  • Cell Proliferation
  • Female
  • Gene Expression Profiling* / methods
  • Gene Expression Regulation, Neoplastic
  • Genotype
  • Melanoma, Experimental / genetics*
  • Melanoma, Experimental / metabolism
  • Melanoma, Experimental / pathology
  • Mice, Inbred C57BL
  • Microphthalmia-Associated Transcription Factor / genetics
  • Microphthalmia-Associated Transcription Factor / metabolism
  • Phenotype
  • Protein Precursors / genetics
  • Protein Precursors / metabolism
  • Proto-Oncogene Proteins c-myc / genetics
  • Proto-Oncogene Proteins c-myc / metabolism
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Skin Neoplasms / genetics*
  • Skin Neoplasms / metabolism
  • Skin Neoplasms / pathology
  • Thymosin / analogs & derivatives
  • Thymosin / genetics
  • Thymosin / metabolism
  • Time Factors
  • Tumor Burden


  • Biomarkers, Tumor
  • CRD-BP protein, mouse
  • Microphthalmia-Associated Transcription Factor
  • Mitf protein, mouse
  • Myc protein, mouse
  • Protein Precursors
  • Proto-Oncogene Proteins c-myc
  • RNA-Binding Proteins
  • prothymosin alpha
  • Thymosin