Inhibition of neutrophil adherence to endothelial cells by 3-deazaadenosine

Agents Actions. 1989 Jun;27(3-4):398-400. doi: 10.1007/BF01972833.

Abstract

Treatment of cultured human umbilical vein endothelial cells (HUVE) with tumor necrosis factor alpha (TNF-alpha) increases their capacity to adhere human neutrophils. We have found that 3-deazaadenosine (c3Ado), when added in conjunction with TNF-alpha, inhibited this increase in neutrophil adherence. This activity of c3Ado was potentiated by the addition of L-homocysteine thiolactone (Hcy). The ability of c3Ado to inhibit neutrophil adherence to HUVE may contribute to the anti-inflammatory activity of this nucleoside analogue.

MeSH terms

  • Aminoglycosides
  • Anti-Bacterial Agents / pharmacology*
  • Cells, Cultured
  • Endothelium, Vascular / cytology*
  • Humans
  • In Vitro Techniques
  • Leukocyte Adherence Inhibition Test
  • Neutrophils / drug effects*
  • Tubercidin / pharmacology*
  • Tumor Necrosis Factor-alpha / pharmacology
  • Umbilical Veins / cytology

Substances

  • Aminoglycosides
  • Anti-Bacterial Agents
  • Tumor Necrosis Factor-alpha
  • 3-deazaadenosine
  • Tubercidin