Molecular and Structural Changes Induced by Essential Oil Treatments in Vicia Faba Roots Detected by FTIR and FTNIR Spectroscopy

Environ Sci Pollut Res Int. 2017 Feb;24(6):5305-5315. doi: 10.1007/s11356-016-8232-6. Epub 2016 Dec 24.

Abstract

Essential oils have recognized antimicrobial and antifungal properties which allow their utilization in agriculture like an alternative to pesticides, but their utilization requires the knowledge of all the potential structural changes and damages produced by the interaction with the vegetal organisms. In this paper, we investigated the effects of two essential oils, the tea tree oil (TTO) and the mixture of clove and rosemary oils (C + R), on the molecular structure of Vicia faba roots by Fourier transform infrared (FTIR) and Fourier near infrared transform (FTNIR) spectroscopy. FTIR spectroscopy showed structural modifications of the absorption bands related to DNA (1100 and 1050 cm-1, carbohydrate backbones, and nucleotide bands within 900 and 850 cm-1), proteins (1700 and 1600 cm-1 amide I band, 1580 and 1520 cm-1 amide II band), and lipids (methylene group of aliphatic chains between 2950 and 2800 cm-1). The changes in the secondary structures of proteins consisted of a denaturation depending on increased presence of random coil structures. In addition, in the samples treated with TTO oils, we observed the presence of protein oxidation, an effect negligible instead for the C + R-treated samples. The modified shapes of the infrared methyl bands of aliphatic chains suggested an increased lipid disorder which could also determine lipid peroxidation. The changes observed for the DNA structures at the highest concentration of the above essential oils can be related to the genotoxic effect of eugenol, an important constituent of both TTO and C + R mixture oils. FTNIR spectroscopy showed the modified shape of the second overtone bands belonging to methyl and methylene groups, between 8500 and 8000 cm-1. This confirmed the increased lipid disorders already observed by FTIR spectroscopy. The results obtained on the probe organism V. faba show that FTIR and FTNIR spectroscopy can become a useful support to the conventional cytogenetic tests used in the evaluation of the allelopathic uses of essential oils in agriculture.

Keywords: DNA modifications; Denaturation of proteins; Essential oils; FTIR spectroscopy; FTNIR spectroscopy; Lipid modifications; Protein oxidation.

MeSH terms

  • Anti-Infective Agents
  • Carbohydrates
  • Lipids
  • Oils, Volatile*
  • Oxidation-Reduction
  • Plant Oils / chemistry*
  • Plant Roots / drug effects
  • Protein Structure, Secondary
  • Proteins
  • Spectroscopy, Fourier Transform Infrared / methods
  • Vicia faba*

Substances

  • Anti-Infective Agents
  • Carbohydrates
  • Lipids
  • Oils, Volatile
  • Plant Oils
  • Proteins