Using Confocal Microscopy and Computational Modeling to Investigate the Cell-Penetrating Properties of Antimicrobial Peptides

Methods Mol Biol. 2017:1548:191-199. doi: 10.1007/978-1-4939-6737-7_13.


Antimicrobial peptides (AMPs) may display the ability to penetrate cells, which may be relevant for their antibiotic activity. To investigate the relevance of the penetrating activity for the antibiotic activity of AMPs, here we describe a method based on the combined use of confocal microscopy and computational modeling coupled with cell death kinetics.

Keywords: Antimicrobial peptides; Cell death kinetics; Cell-penetrating peptides; Confocal microscopy; Mathematical modeling; Multifunctional peptides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Anti-Infective Agents / chemistry*
  • Anti-Infective Agents / metabolism
  • Antimicrobial Cationic Peptides / chemistry*
  • Antimicrobial Cationic Peptides / metabolism
  • Cell-Penetrating Peptides / chemistry*
  • Cell-Penetrating Peptides / metabolism
  • Computer Simulation
  • Fluorescent Dyes
  • Microscopy, Confocal*
  • Models, Theoretical*
  • Staining and Labeling


  • Anti-Infective Agents
  • Antimicrobial Cationic Peptides
  • Cell-Penetrating Peptides
  • Fluorescent Dyes