Isolation, purification, and partial characterization of prunellin, an anti-HIV component from aqueous extracts of Prunella vulgaris

H D Tabba; R S Chang; K M Smith

doi:10.1016/0166-3542(89)90036-3

Isolation, purification, and partial characterization of prunellin, an anti-HIV component from aqueous extracts of Prunella vulgaris

Antiviral Res. 1989 Jun-Jul;11(5-6):263-73. doi: 10.1016/0166-3542(89)90036-3.

Authors

H D Tabba¹, R S Chang, K M Smith

Affiliation

¹ Department of Chemistry, University of California, Davis 95616.

PMID: 2802570
DOI: 10.1016/0166-3542(89)90036-3

Abstract

Prunellin, an anti-HIV active compound, was isolated from aqueous extracts of the Chinese medicinal herb, Prunella vulgaris, and purified to chromatographic homogeneity. Infrared and NMR spectroscopy identified prunellin as a polysaccharide. Elemental analyses, precipitation with calcium(II), barium(II), or 9-aminoacridine suggest a sulfated polysaccharide. Paper chromatography of the exhaustively hydrolyzed material indicates the presence of glucose, galactose, xylose, gluconic acid, galactonic acid and galactosamine as the constituent monosaccharides. The molecular size of prunellin, as determined by gel permeation chromatography and the Squire method on Sephadex G-75, is about 10 kDa.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Antiviral Agents / isolation & purification*
Chemical Phenomena
Chemistry
Drugs, Chinese Herbal / isolation & purification*
HIV / drug effects*
Microbial Sensitivity Tests
Polysaccharides / isolation & purification
Polysaccharides / pharmacology*

Substances

Antiviral Agents
Drugs, Chinese Herbal
Polysaccharides
prunellin