Regulation of BC200 RNA-mediated translation inhibition by hnRNP E1 and E2

FEBS Lett. 2017 Jan;591(2):393-405. doi: 10.1002/1873-3468.12544. Epub 2017 Jan 12.


The long noncoding RNA BC200 (brain cytoplasmic RNA, 200 nucleotides) acts as a translational modulator of local protein synthesis at dendrites. BC200 RNA has been shown to inhibit translation in vitro, but it remains unknown how this translation inhibition might be controlled in a cell. Here, we performed yeast three-hybrid screening and identified hnRNP E1 and hnRNP E2 as BC200 RNA-interacting proteins. We found that: these hnRNA proteins could restore BC200 RNA-inhibited translation; BC200 RNA interacts with hnRNP E1 and E2 mainly through its unique 3' C-rich domain; and the RNA binding specificities and modes of the two proteins differed somewhat. Our results offer new insights into the regulation of BC200 RNA-mediated translation inhibition.

Keywords: BC200 RNA; RNA-protein interaction; hnRNP E1; hnRNP E2; regulation of translation.

Publication types

  • Letter

MeSH terms

  • Base Sequence
  • DNA-Binding Proteins
  • Eukaryotic Initiation Factor-4A / metabolism
  • HeLa Cells
  • Heterogeneous-Nuclear Ribonucleoproteins / metabolism*
  • Humans
  • Nucleic Acid Conformation
  • Protein Binding
  • Protein Biosynthesis*
  • RNA, Long Noncoding / metabolism*
  • RNA-Binding Proteins / metabolism*


  • DNA-Binding Proteins
  • Heterogeneous-Nuclear Ribonucleoproteins
  • PCBP1 protein, human
  • PCBP2 protein, human
  • RNA, Long Noncoding
  • RNA-Binding Proteins
  • long non-coding RNA BC200, human
  • Eukaryotic Initiation Factor-4A