Lacinutrix chionocetis sp. nov., isolated from gut of a red snow crab

Arch Microbiol. 2017 May;199(4):597-603. doi: 10.1007/s00203-016-1330-5. Epub 2016 Dec 27.

Abstract

A Gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated MAB-07T, was isolated from the gut of a red snow crab. The novel strain grew optimally at 20 °C, pH 7.0-8.0, and in the presence of 3% (w/v) NaCl. A phylogenetic analysis based on the 16S rRNA gene sequence indicated that the strain MAB-07T belongs to the type strains of species of the genus Lacinutrix. Strain MAB-07T exhibited 16S rRNA gene sequence similarity values of 95.5-97.8% with the type strains of species of the genus Lacinutrix. The predominant cellular fatty acids of strain MAB-07T were iso-C15:1 G (27.5%) and iso-C15:0 (21.7%). The major respiratory quinine was identified as MK-6. The polar lipids consisted of phosphatidylethanolamine, four unidentified aminolipids, and two unidentified lipids. The genomic DNA G + C content was determined to be 33.3%, and its DNA-DNA relatedness values with the type strains of L. venerupis, L. mariniflava, L. jangbogonensis, L. algicola, and Olleya aquimaris were 28-32%. Based on the data from this polyphasic taxonomic study, strain MAB-07T is considered to represent a novel species of the genus Lacinutrix, for which the name L. chionocetis sp. nov. is proposed. The type strain is MAB-07T (=KCTC 42767T = JCM 30988T).

Keywords: Deep sea area; Flavobacteriaceae; Lacinutrix chionocetis; Red snow crab.

MeSH terms

  • Animals
  • Base Composition
  • Brachyura / microbiology*
  • DNA, Bacterial / chemistry
  • Fatty Acids / analysis
  • Flavobacteriaceae / chemistry
  • Flavobacteriaceae / classification*
  • Flavobacteriaceae / isolation & purification
  • Gastrointestinal Tract / microbiology
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Vitamin K 2 / analogs & derivatives
  • Vitamin K 2 / analysis

Substances

  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • menaquinone 6