Ciprofloxacin-Mediated Mutagenesis Is Suppressed by Subinhibitory Concentrations of Amikacin in Pseudomonas aeruginosa

Antimicrob Agents Chemother. 2017 Feb 23;61(3):e02107-16. doi: 10.1128/AAC.02107-16. Print 2017 Mar.

Abstract

Resistance to antibiotics is a global health problem. Activation of the SOS response, and the subsequent elevation in mutagenesis, contributes to the appearance of resistance mutations. Among currently used drugs, quinolones are the most potent inducers of the SOS response. In the present study, we show that amikacin inhibits ciprofloxacin-mediated SOS induction and mutagenesis in Pseudomonas aeruginosa.

Keywords: Pseudomonas aeruginosa; SOS response; ciprofloxacin; recA.

MeSH terms

  • Amikacin / pharmacology*
  • Anti-Bacterial Agents / pharmacology
  • Ciprofloxacin / antagonists & inhibitors
  • Ciprofloxacin / pharmacology*
  • Disk Diffusion Antimicrobial Tests
  • Gene Expression
  • Genes, Reporter
  • Luciferases / genetics
  • Luciferases / metabolism
  • Mutagenesis / drug effects
  • Mutation*
  • Plasmids / chemistry
  • Plasmids / metabolism
  • Pseudomonas aeruginosa / drug effects*
  • Pseudomonas aeruginosa / genetics
  • Pseudomonas aeruginosa / growth & development
  • Rec A Recombinases / genetics*
  • Rec A Recombinases / metabolism
  • SOS Response, Genetics / drug effects

Substances

  • Anti-Bacterial Agents
  • Ciprofloxacin
  • Amikacin
  • Luciferases
  • Rec A Recombinases