PER-8, a Novel Extended-Spectrum β-Lactamase PER Variant, from an Acinetobacter baumannii Clinical Isolate in Nepal

Antimicrob Agents Chemother. 2017 Feb 23;61(3):e02300-16. doi: 10.1128/AAC.02300-16. Print 2017 Mar.

Abstract

A novel PER-type extended-spectrum β-lactamase, PER-8, was identified in an Acinetobacter baumannii clinical isolate obtained in Nepal. The amino acid sequence of PER-8 has a substitution at position 39 (Gly to Glu) compared with that of PER-7. The kcat/Km ratio of PER-8 for aztreonam was lower than that of PER-7, while the kcat/Km ratio of PER-8 for imipenem was higher than that of PER-7. The genomic environment surrounding blaPER-8 was intI1 blaPSE-1qacEDI sulI ISCR1-blaPER-8gts sulI orfX on a 100-kb plasmid.

Keywords: Acinetobacter baumannii; PER-type ESBLs; plasmid-mediated resistance.

MeSH terms

  • Acinetobacter Infections / epidemiology
  • Acinetobacter Infections / microbiology
  • Acinetobacter baumannii / drug effects
  • Acinetobacter baumannii / genetics*
  • Acinetobacter baumannii / growth & development
  • Acinetobacter baumannii / isolation & purification
  • Amino Acid Sequence
  • Amino Acid Substitution*
  • Anti-Bacterial Agents / pharmacology
  • Aztreonam / pharmacology
  • Drug Resistance, Multiple, Bacterial / genetics*
  • Humans
  • Imipenem / pharmacology
  • Kinetics
  • Microbial Sensitivity Tests
  • Nepal / epidemiology
  • Plasmids / chemistry
  • Plasmids / metabolism
  • beta-Lactamases / genetics*
  • beta-Lactamases / metabolism

Substances

  • Anti-Bacterial Agents
  • Imipenem
  • beta-Lactamases
  • Aztreonam