Abstract
A novel PER-type extended-spectrum β-lactamase, PER-8, was identified in an Acinetobacter baumannii clinical isolate obtained in Nepal. The amino acid sequence of PER-8 has a substitution at position 39 (Gly to Glu) compared with that of PER-7. The kcat/Km ratio of PER-8 for aztreonam was lower than that of PER-7, while the kcat/Km ratio of PER-8 for imipenem was higher than that of PER-7. The genomic environment surrounding blaPER-8 was intI1 blaPSE-1qacEDI sulI ISCR1-blaPER-8gts sulI orfX on a 100-kb plasmid.
Keywords:
Acinetobacter baumannii; PER-type ESBLs; plasmid-mediated resistance.
Copyright © 2017 American Society for Microbiology.
MeSH terms
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Acinetobacter Infections / epidemiology
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Acinetobacter Infections / microbiology
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Acinetobacter baumannii / drug effects
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Acinetobacter baumannii / genetics*
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Acinetobacter baumannii / growth & development
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Acinetobacter baumannii / isolation & purification
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Amino Acid Sequence
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Amino Acid Substitution*
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Anti-Bacterial Agents / pharmacology
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Aztreonam / pharmacology
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Drug Resistance, Multiple, Bacterial / genetics*
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Humans
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Imipenem / pharmacology
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Kinetics
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Microbial Sensitivity Tests
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Nepal / epidemiology
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Plasmids / chemistry
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Plasmids / metabolism
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beta-Lactamases / genetics*
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beta-Lactamases / metabolism
Substances
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Anti-Bacterial Agents
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Imipenem
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beta-Lactamases
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Aztreonam