Rapid Naked-Eye Discrimination of Cytochrome P450 Genetic Polymorphism through Non-Crosslinking Aggregation of DNA-Functionalized Gold Nanoparticles

Yoshitsugu Akiyama; Guoqing Wang; Shota Shiraishi; Naoki Kanayama; Tohru Takarada; Mizuo Maeda

doi:10.1002/open.201600142

Rapid Naked-Eye Discrimination of Cytochrome P450 Genetic Polymorphism through Non-Crosslinking Aggregation of DNA-Functionalized Gold Nanoparticles

ChemistryOpen. 2016 Nov 15;5(6):507. doi: 10.1002/open.201600142. eCollection 2016 Dec.

Authors

Yoshitsugu Akiyama¹, Guoqing Wang², Shota Shiraishi², Naoki Kanayama³, Tohru Takarada², Mizuo Maeda²

Affiliations

¹ Bioengineering Laboratory RIKEN2-1 Hirosawa, Wako Saitama 351-0198 Japan; Faculty of Industrial Science and Technology Tokyo University of Science 102-1 Tomino, Oshamambe-cho, Yamakoshi-gun Hokkaido 049-3514 Japan.
² Bioengineering Laboratory RIKEN 2-1 Hirosawa, Wako Saitama 351-0198 Japan.
³ Bioengineering Laboratory RIKEN2-1 Hirosawa, Wako Saitama 351-0198 Japan; Interdisciplinary Graduate School of Science and Technology Shinshu University 4-7-1 Wakasato, Nagano-shi Nagano 380-8553 Japan.

Abstract

The front cover artwork is provided by the group of Tohru Takarada at RIKEN (Japan). The image shows a colorimetric single-nucleotide polymorphism (SNP) genotyping method that uses spontaneous aggregation of DNA-modified gold nanoparticles (DNA-AuNPs) for the simple and rapid SNP genotyping of the human cytochrome P450 2C19 monooxygenase gene. For more details, read the full text of the Full Paper at p. 508.