The acyltransferase LYCAT controls specific phosphoinositides and related membrane traffic

Mol Biol Cell. 2017 Jan 1;28(1):161-172. doi: 10.1091/mbc.E16-09-0668. Epub 2016 Nov 9.


Phosphoinositides (PIPs) are key regulators of membrane traffic and signaling. The interconversion of PIPs by lipid kinases and phosphatases regulates their functionality. Phosphatidylinositol (PI) and PIPs have a unique enrichment of 1-stearoyl-2-arachidonyl acyl species; however, the regulation and function of this specific acyl profile remains poorly understood. We examined the role of the PI acyltransferase LYCAT in control of PIPs and PIP-dependent membrane traffic. LYCAT silencing selectively perturbed the levels and localization of phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] and phosphatidylinositol-3-phosphate and the membrane traffic dependent on these specific PIPs but was without effect on phosphatidylinositol-4-phosphate or biosynthetic membrane traffic. The acyl profile of PI(4,5)P2 was selectively altered in LYCAT-deficient cells, whereas LYCAT localized with phosphatidylinositol synthase. We propose that LYCAT remodels the acyl chains of PI, which is then channeled into PI(4,5)P2 Our observations suggest that the PIP acyl chain profile may exert broad control of cell physiology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1-Acylglycerol-3-Phosphate O-Acyltransferase / metabolism*
  • 1-Acylglycerol-3-Phosphate O-Acyltransferase / physiology*
  • Acyltransferases / metabolism
  • Acyltransferases / physiology
  • Cell Culture Techniques
  • Cell Line
  • Cell Membrane / metabolism
  • Humans
  • Phosphatidylinositol Phosphates / metabolism
  • Phosphatidylinositols / chemistry
  • Phosphatidylinositols / metabolism*
  • Phosphatidylinositols / physiology
  • Phosphoric Monoester Hydrolases / metabolism
  • Phosphotransferases
  • Protein Transport / physiology
  • Retinal Pigment Epithelium


  • Phosphatidylinositol Phosphates
  • Phosphatidylinositols
  • phosphatidylinositol 3-phosphate
  • phosphatidylinositol 4-phosphate
  • Acyltransferases
  • 1-Acylglycerol-3-Phosphate O-Acyltransferase
  • LCLAT1 protein, human
  • Phosphotransferases
  • Phosphoric Monoester Hydrolases

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