Proteomic Analysis of Kidney Preservation Solutions Prior to Renal Transplantation

PLoS One. 2016 Dec 30;11(12):e0168755. doi: 10.1371/journal.pone.0168755. eCollection 2016.


One of the main issues in kidney transplantation is the optimal functional preservation of the organ until its transplantation into the appropriate recipient. Despite intensive efforts, the functional preservation period remains limited to hours. During this time, as a result of cellular injury, various proteins, peptides, and other molecules are released by the organ into the preservation medium. In this study, we used proteomic techniques to analyze the protein profiles of preservation solutions in which organs had been preserved prior to their transplantation. Samples were obtained from the preservation solutions of 25 deceased donor kidneys scheduled for transplantation. The protein profiles of the solutions were analyzed using 2D gel electrophoresis/MALDI-TOF and LC-MS/MS. We identified and quantified 206 proteins and peptides belonging to 139 different groups. Of these, 111 proteins groups were belonging to kidney tissues. This study used proteomic techniques to analyze the protein profiles of organ preservation solutions. These findings will contribute to the development of improved preservation solutions to effectively protect organs for transplantation.

MeSH terms

  • Chromatography, Liquid / methods
  • Kidney / metabolism*
  • Kidney Transplantation / methods
  • Organ Preservation / methods
  • Organ Preservation Solutions / metabolism*
  • Peptides / metabolism
  • Proteins / metabolism
  • Proteomics / methods
  • Tandem Mass Spectrometry / methods


  • Organ Preservation Solutions
  • Peptides
  • Proteins

Grants and funding

This study was financed by The Scientific and Technological Research Council of Turkey (TUBITAK), project number 110S103, The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.