The NLRP3 inflammasome is a potential target of ozone therapy aiming to ease chronic renal inflammation in chronic kidney disease

Int Immunopharmacol. 2017 Feb;43:203-209. doi: 10.1016/j.intimp.2016.12.022. Epub 2016 Dec 27.


Ozone therapy is an effective medical treatment for various diseases. A previous study has demonstrated its reno-protective effect in chronic kidney disease (CKD), but the mechanism involved is not completely known. This study produced the 5/6 nephrectomized CKD rat model and investigated whether the reno-protective effect of ozone therapy was achieved by its anti-inflammatory property through the modulation of the NLRP3 inflammasome. The results showed that ozone therapy at a low concentration improved renal function and ameliorated renal morphological injury in 5/6 nephrectomized rats. The expression of NLRP3, ASC, and caspase-1-p10 in the kidney of these rats was simultaneously lowered by ozone therapy. Moreover, renal inflammation caused by IL-1β was significantly alleviated by ozone therapy. The Pearson correlation analysis indicated that the protein level of IL-1β was positively correlated with renal injury scores. Taken together, these results indicated that ozone therapy might reduce sterile renal inflammation and slow down CKD progression through the modulation of the NLRP3 inflammasome in 5/6 nephrectomized rats.

Keywords: CKD; IL-1β; NLRP3; Ozone therapy; Renal inflammation.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / therapeutic use*
  • Apoptosis Regulatory Proteins / metabolism
  • CARD Signaling Adaptor Proteins
  • Caspase 1 / metabolism
  • Disease Models, Animal
  • Humans
  • Inflammasomes / metabolism*
  • Kidney / drug effects*
  • Kidney / immunology
  • Male
  • NLR Family, Pyrin Domain-Containing 3 Protein / metabolism*
  • Nephrectomy
  • Ozone / therapeutic use*
  • Rats
  • Rats, Sprague-Dawley
  • Renal Insufficiency, Chronic / drug therapy*


  • Anti-Inflammatory Agents
  • Apoptosis Regulatory Proteins
  • CARD Signaling Adaptor Proteins
  • Inflammasomes
  • NLR Family, Pyrin Domain-Containing 3 Protein
  • Nlrp3 protein, rat
  • Pycard protein, rat
  • Ozone
  • Caspase 1