TGFβ1 induces stress fiber formation through upregulation of TRPC6 in vascular smooth muscle cells

Biochem Biophys Res Commun. 2017 Jan 29;483(1):129-134. doi: 10.1016/j.bbrc.2016.12.179. Epub 2016 Dec 27.

Abstract

Aberrant transforming growth factor β1 (TGFβ1) signaling plays a crucial role in the pathogenesis of vascular fibrosis. On the other hand, deregulated transient receptor potential canonical 6 (TRPC6) channel expression shows impaired vascular physiology and wound healing. However, it has little been known about the functional association between TGFβ1 and TRPC6 in vascular smooth muscle cells (VSMCs). In this study, we analyzed the microarray data obtained from TGFβ1-treated A7r5 VSMCs. We found that TGFβ1 specifically elevates the expression level of TRPC6 mainly through Smad-dependent canonical pathway. The siRNA against TRPC6 abolished TGFβ1-induced molecular and cellular phenotype changes, including myosin light chain phosphorylation, actin stress fiber formation, and cell migration. These results demonstrate that TRPC6 is an important component of TGFβ1 signaling pathway in VSMCs. Therefore, our findings provide a basis for future investigation aimed at developing novel therapeutic strategies for treatment of vascular fibrosis.

Keywords: Fibrosis; Stress fiber; TGFβ1; TRPC6; Vascular smooth muscle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism
  • Animals
  • Cell Line
  • Cell Movement
  • Fibrosis
  • Muscle, Smooth, Vascular / metabolism*
  • Muscle, Smooth, Vascular / pathology
  • Myocytes, Smooth Muscle / metabolism
  • Myocytes, Smooth Muscle / pathology
  • Myosin Light Chains / metabolism
  • Phosphorylation
  • RNA, Small Interfering / genetics
  • Rats
  • Signal Transduction
  • Smad Proteins / metabolism
  • Stress Fibers / metabolism*
  • Stress Fibers / pathology
  • TRPC Cation Channels / antagonists & inhibitors
  • TRPC Cation Channels / genetics
  • TRPC Cation Channels / metabolism*
  • Transforming Growth Factor beta1 / metabolism*
  • Up-Regulation

Substances

  • Actins
  • Myosin Light Chains
  • RNA, Small Interfering
  • Smad Proteins
  • TRPC Cation Channels
  • Transforming Growth Factor beta1
  • Trpc6 protein, rat