Acetyl-CoA production by encapsulated pyruvate ferredoxin oxidoreductase in alginate hydrogels

Makoto Takenaka; Ki-Seok Yoon; Takahiro Matsumoto; Seiji Ogo

doi:10.1016/j.biortech.2016.12.051

Acetyl-CoA production by encapsulated pyruvate ferredoxin oxidoreductase in alginate hydrogels

Bioresour Technol. 2017 Mar:227:279-285. doi: 10.1016/j.biortech.2016.12.051. Epub 2016 Dec 18.

Authors

Makoto Takenaka¹, Ki-Seok Yoon², Takahiro Matsumoto², Seiji Ogo³

Affiliations

¹ International Institute for Carbon-Neutral Energy Research (WPI-I(2)CNER), Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan; Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.
² International Institute for Carbon-Neutral Energy Research (WPI-I(2)CNER), Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan; Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan; Centre for Small Molecule Energy, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.
³ International Institute for Carbon-Neutral Energy Research (WPI-I(2)CNER), Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan; Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan; Centre for Small Molecule Energy, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan. Electronic address: ogo.seiji.872@m.kyushu-u.ac.jp.

PMID: 28040649
DOI: 10.1016/j.biortech.2016.12.051

Abstract

Pyruvate ferredoxin oxidoreductase from Citrobacter sp. S-77 (PFOR_S77) was purified in order to develop a method for acetyl-CoA production. Although the purified PFOR_S77 showed high O₂-sensitivity, the activity could be remarkably stabilized in anaerobic conditions. PFOR_S77 was effectively immobilized on ceramic hydroxyapatite (PFOR_S77-HA) with an efficiency of more than 96%, however, after encapsulation of PFOR_S77-HA in alginate, the rate of catalytic acetyl-CoA production was highly reduced to 36% when compared to that of the free enzyme. However, the operational stability of the PFOR_S77-HA in alginate hydrogels was remarkable, retaining over 68% initial activity even after ten repeated cycles. The results suggested that the PFOR_S77-HA hydrogels have a high potential for biotechnological application.

Keywords: Acetyl-CoA production; Encapsulation; Immobilization; Kinetic parameters; Purification and characterization; Pyruvate ferredoxin oxidoreductase.

MeSH terms

Acetyl Coenzyme A / chemical synthesis*
Alginates / chemistry*
Catalysis
Citrobacter / enzymology
Glucuronic Acid / chemistry
Hexuronic Acids / chemistry
Hydrogels / chemistry*
Pyruvate Synthase / chemistry*
Pyruvate Synthase / metabolism

Substances

Alginates
Hexuronic Acids
Hydrogels
Acetyl Coenzyme A
Glucuronic Acid
Pyruvate Synthase