A far-red emitting fluorescent marker protein, mGarnet2, for microscopy and STED nanoscopy

Chem Commun (Camb). 2017 Jan 10;53(5):979-982. doi: 10.1039/c6cc09081h.

Abstract

Here we present mGarnet2, a monomeric, far-red fluorescent marker protein derived from mRuby, with absorption and emission bands peaking at 598 and 671 nm, respectively. The protein shows excellent performance as a live-cell fusion marker for STED nanoscopy with 640 nm excitation and 780 nm depletion wavelengths.

MeSH terms

  • Luminescent Proteins / chemistry*
  • Microscopy, Fluorescence
  • Nanotechnology*

Substances

  • Luminescent Proteins
  • red fluorescent protein