Intrinsic blinking of red fluorescent proteins for super-resolution microscopy

Chem Commun (Camb). 2017 Jan 10;53(5):949-951. doi: 10.1039/c6cc09200d.

Abstract

Single-molecule localization microscopy relies on either controllable photoswitching of fluorescent probes or their robust blinking. We have found that blinking of monomeric red fluorescent proteins TagRFP, TagRFP-T, and FusionRed occurs at moderate illumination power and matches well with camera acquisition speed. It allows for super-resolution image reconstruction of densely labelled structures in live cells using various algorithms.

MeSH terms

  • Algorithms
  • HeLa Cells
  • Humans
  • Luminescent Proteins / chemistry*
  • Microscopy, Fluorescence

Substances

  • Luminescent Proteins
  • red fluorescent protein