Expression of epithelial cell-derived cytokine genes in the duodenal and colonic mucosae of dogs with chronic enteropathy

J Vet Med Sci. 2017 Feb 28;79(2):393-397. doi: 10.1292/jvms.16-0451. Epub 2016 Dec 29.


It remains unclear whether epithelial cell-derived cytokines, including interleukin (IL)-25, IL-33 and thymic stromal lymphopoietin (TSLP), contribute to development of canine chronic enteropathy (CE), which includes antibiotic-responsive enteropathy (ARE), food-responsive enteropathy (FRE) and inflammatory bowel disease (IBD). In the present study, we examined mRNA expression of il-25, il-33 and tslp in the duodenal and colonic mucosae of dogs with ARE, FRE and IBD. Real-time PCR analysis revealed that mRNA expression of il-33 was significantly lower in the duodenum in dogs with FRE than in healthy dogs. The results suggest that epithelial cell-derived cytokines may not be an inducer of Th2-type immunity in the gut of dogs with CE, and decreased expression of IL-33 may be involved in induction of FRE. Further studies are required to clarify roles of epithelial cell-derived cytokines, especially IL-33, in the pathogenesis of canine CE.

MeSH terms

  • Animals
  • Chronic Disease
  • Colon / metabolism*
  • Cytokines / genetics*
  • Cytokines / metabolism
  • Dog Diseases / genetics
  • Dog Diseases / metabolism*
  • Dogs
  • Duodenum / metabolism*
  • Interleukin-17 / genetics
  • Interleukin-17 / metabolism
  • Interleukin-33 / genetics
  • Interleukin-33 / metabolism
  • Intestinal Diseases / genetics
  • Intestinal Diseases / metabolism
  • Intestinal Diseases / veterinary*
  • Intestinal Mucosa / metabolism*
  • Male
  • RNA, Messenger / metabolism


  • Cytokines
  • Interleukin-17
  • Interleukin-33
  • RNA, Messenger
  • thymic stromal lymphopoietin