Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 2016, 9238512

Effects of Extracts From Tiaozhi Granule and Its Components on Expression of Scavenger Receptor Class B Type I

Affiliations

Effects of Extracts From Tiaozhi Granule and Its Components on Expression of Scavenger Receptor Class B Type I

Xiao Yu et al. Evid Based Complement Alternat Med.

Abstract

Sera from the rats with different drug treatments (atorvastatin, Tiaozhi granule, or its extracts) were collected. LO-2 cells or HepG2 cells were pretreated with different sera as the following groups randomly: (1) blank control group, (2) positive control group (atorvastatin group), (3) Tiaozhi granule water extract groups, (4) Tiaozhi granule alcohol extract groups, and (5) alcohol extracts for each component: Pollen Typhae Angustifoliae, Curcuma longa L., and Rhizoma Alismatis. LO-2 cells were cotransfected with plasmid carrying SR-BI and pRL-TK promoter genes. Promoter activity was measured by the luciferase reporter gene assay. The mRNA and protein expressions of SR-BI were examined using real-time PCR and western blot analyses. Our results show that promoter activity and mRNA and protein expression levels of the SR-BI were significantly upregulated by Tiaozhi granules alcohol or water extracts in a dose-dependent manner. Pollen Typhae Angustifoliae alcohol extract with a high dosage could also increase SR-BI activity and expression, but not the extracts from Curcuma longa L. and Rhizoma Alismatis. Both Tiaozhi granule alcohol and water extracts can upregulate SR-BI gene expression. Among the components, Pollen Typhae Angustifoliae are important for the regulatory effect coordinating with Curcuma longa L. and Rhizoma Alismatis.

Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Figure 1
Figure 1
Effects of Tiaozhi granule alcohol extract on SR-BI expression in LO-2 cells. (a) Effect of Tiaozhi granule alcohol extract on SR-BI promoter activity (n = 12). (b) Effect of Tiaozhi granule alcohol extract on SR-BI mRNA expression (n = 6). (c) Effect of Tiaozhi granule alcohol extract on SR-BI protein expression (n = 6). The upper panels are representative blot of SR-BI and GAPDH; the lower panel is the densitometric analysis of SR-BI expression normalized to GAPDH. Data were presented as mean ± SEM. P < 0.05 compared with control group, Δ P < 0.05 compared with atorvastatin group, and P < 0.05 compared with high and medium group.
Figure 2
Figure 2
Effects of Tiaozhi granule water extract on SR-BI expression in LO-2 cells. (a) Effect of Tiaozhi granule water extract on SR-BI promoter activity (n = 12). (b) Effect of Tiaozhi granule water extract on SR-BI mRNA expression (n = 6). (c) Effect of Tiaozhi granule water extract on SR-BI protein expression (n = 6). The upper panels are representative blot of SR-BI and GAPDH; the lower panel is the densitometric analysis of SR-BI expression normalized to GAPDH. Data are presented as mean ± SEM. P < 0.05 compared with control group, Δ P < 0.05 compared with atorvastatin group, and P < 0.05 compared with high and medium groups.
Figure 3
Figure 3
Effects of Pollen Typhae Angustifoliae alcohol extract on SR-BI expression in LO-2 cells. (a) Effect of Pollen Typhae Angustifoliae alcohol extract on SR-BI promoter activity (n = 12). (b) Effect of Pollen Typhae Angustifoliae alcohol extract on SR-BI mRNA expression (n = 6). (c) Effect of Pollen Typhae Angustifoliae alcohol extract on SR-BI protein expression (n = 6). The upper panels are representative blot of SR-BI and GAPDH; the lower panel is the densitometric analysis of SR-BI expression normalized to GAPDH. Data are presented as mean ± SEM. P < 0.05 compared with control group, Δ P < 0.05 compared with atorvastatin group, and P < 0.05 compared with high dose of Tiaozhi granule alcohol extract group.
Figure 4
Figure 4
Effects of Curcuma longa L. alcohol extract on SR-BI expression in LO-2 cells. (a) Effect of Curcuma longa L. alcohol extract on SR-BI promoter activity (n = 12). (b) Effect of Curcuma longa L. alcohol extract on SR-BI mRNA expression (n = 6). (c) Effect of Curcuma longa L. extract on SR-BI protein expression (n = 6). The upper panels are representative blot of SR-BI and GAPDH; the lower panel is the densitometric analysis of SR-BI expression normalized to GAPDH. Data are presented as mean ± SEM. P < 0.05 compared with control group, Δ P < 0.05 compared with atorvastatin group, and P < 0.05 compared with high dose of Tiaozhi granule alcohol extract group.
Figure 5
Figure 5
Effects of Rhizoma Alismatis alcohol extract on SR-BI expression in LO-2 cells. (a) Effect of Rhizoma Alismatis alcohol extract on SR-BI promoter activity (n = 12). (b) Effect of Rhizoma Alismatis alcohol extract on SR-BI mRNA expression (n = 6). (c) Effect of Rhizoma Alismatis alcohol extract on SR-BI protein expression (n = 6). The upper panels are representative blot of SR-BI and GAPDH; the lower panel is the densitometric analysis of SR-BI expression normalized to GAPDH. Data are presented as mean ± SEM. P < 0.05 compared with control group, Δ P < 0.05 compared with atorvastatin group, and P < 0.05 compared with high dose of Tiaozhi granule alcohol extract group.
Figure 6
Figure 6
Effects of Tiaozhi granule alcohol extract and Pollen Typhae Angustifoliae alcohol extract on SR-BI expression in HepG2 cells. (a) Effects of Tiaozhi granule alcohol extract and Pollen Typhae Angustifoliae alcohol extract on SR-BI promoter activity (n = 12). (b) Effects of Tiaozhi granule alcohol extract and Pollen Typhae Angustifoliae alcohol extract on SR-BI mRNA expression (n = 6). (c) Effects of Tiaozhi granule alcohol extract and Pollen Typhae Angustifoliae alcohol extract on SR-BI protein expression (n = 6). The upper panels are representative blot of SR-BI and GAPDH; the lower panel is the densitometric analysis of SR-BI expression normalized to GAPDH. Data are presented as mean ± SEM. P < 0.05 compared with control group, Δ P < 0.05 compared with atorvastatin group, and P < 0.05 compared with high dose of Tiaozhi granule alcohol extract group.

Similar articles

See all similar articles

Cited by 1 article

References

    1. Fisher E. A. Regression of atherosclerosis: the journey from the liver to the plaque and back. Arteriosclerosis, Thrombosis, and Vascular Biology. 2016;36(2):226–232. doi: 10.1161/atvbaha.115.301926. - DOI - PMC - PubMed
    1. Hegele R. A., Gidding S. S., Ginsberg H. N., et al. Nonstatin Low-Density Lipoprotein-Lowering Therapy and Cardiovascular Risk Reduction-Statement from ATVB Council. Arteriosclerosis, Thrombosis, and Vascular Biology. 2015;35(11):2269–2280. doi: 10.1161/atvbaha.115.306442. - DOI - PMC - PubMed
    1. Spence J. D. Recent advances in pathogenesis, assessment, and treatment of atherosclerosis. F1000Research. 2016;5 doi: 10.12688/f1000research.8459.1. - DOI - PMC - PubMed
    1. Robinson J. G., Stone N. J. The 2013 ACC/AHA guideline on the treatment of blood cholesterol to reduce atherosclerotic cardiovascular disease risk: a new paradigm supported by more evidence. European Heart Journal. 2015;36(31):2110–2118. doi: 10.1093/eurheartj/ehv182. - DOI - PubMed
    1. Lloyd-Jones D. M., Goff D. C., Stone N. J. Treatment of blood cholesterol to reduce risk for atherosclerotic cardiovascular disease. Annals of Internal Medicine. 2016;164(2):135–136. doi: 10.7326/L15-0520. - DOI - PubMed
Feedback