The murine interleukin-4 receptor: molecular cloning and characterization of secreted and membrane bound forms

Cell. 1989 Oct 20;59(2):335-48. doi: 10.1016/0092-8674(89)90295-x.


Receptors for interleukin-4 (IL-4) are expressed at low levels on a wide variety of primary cells and cultured cell lines. Fluorescence-activated sorting of CTLL-2 cells resulted in the isolation of a subclone, CTLL 19.4, which expressed 10(6) IL-4 receptors per cell. These cells were used for the purification of IL-4 receptor protein and to prepare a hybrid-subtracted cDNA probe for isolation of cDNA clones. Three classes of IL-4 receptor cDNA were identified. The first encoded a 140 kd membrane bound IL-4 receptor containing extracellular, transmembrane, and cytoplasmic domains. The second class lacked the cytoplasmic region, and the third encoded a secreted form of the receptor. All cDNA clones expressed in COS-7 cells had IL-4 binding properties comparable to the native IL-4 receptor. The soluble form of the IL-4 receptor blocked the ability of IL-4 to induce CTLL cell proliferation and may represent a regulatory molecule specific for IL-4-dependent immune responses.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cell Membrane / immunology
  • Cloning, Molecular* / methods
  • DNA / genetics
  • DNA / isolation & purification
  • Flow Cytometry
  • Interleukin-4 / metabolism*
  • Kinetics
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Receptors, Interleukin-4
  • Receptors, Mitogen / biosynthesis
  • Receptors, Mitogen / genetics*
  • Receptors, Mitogen / metabolism
  • Recombinant Proteins / metabolism
  • Restriction Mapping
  • T-Lymphocytes / immunology


  • Receptors, Interleukin-4
  • Receptors, Mitogen
  • Recombinant Proteins
  • Interleukin-4
  • DNA

Associated data

  • GENBANK/M27959
  • GENBANK/M27960