Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo

Sci Rep. 2017 Jan 5:7:39869. doi: 10.1038/srep39869.

Abstract

Salidriside (SDS), a phenylpropanoid glycoside derived from Rhodiola rosea L, has been shown to be neuroprotective in many studies, which may be promising in nerve recovery. In this study, the neuroprotective effects of SDS on engineered nerve constructed by Schwann cells (SCs) and Poly (lactic-co-glycolic acid) (PLGA) were studied in vitro. We further investigated the effect of combinational therapy of SDS and PLGA/SCs based tissue engineering on peripheral nerve regeneration based on the rat model of nerve injury by sciatic transection. The results showed that SDS dramatically enhanced the proliferation and function of SCs. The underlying mechanism may be that SDS affects SCs growth through the modulation of neurotrophic factors (BDNF, GDNF and CNTF). 12 weeks after implantation with a 12 mm gap of sciatic nerve injury, SDS-PLGA/SCs achieved satisfying outcomes of nerve regeneration, as evidenced by morphological and functional improvements upon therapy by SDS, PLGA/SCs or direct suture group assessed by sciatic function index, nerve conduction assay, HE staining and immunohistochemical analysis. Our results demonstrated the significant role of introducing SDS into neural tissue engineering to promote nerve regeneration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cells, Cultured
  • Glucosides / pharmacology*
  • Lactic Acid / chemistry
  • Nerve Growth Factors / metabolism
  • Nerve Regeneration*
  • Neuroprotective Agents / pharmacology*
  • Peripheral Nerve Injuries / therapy*
  • Phenols / pharmacology*
  • Polyglycolic Acid / chemistry
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Rats
  • Rats, Sprague-Dawley
  • Schwann Cells / drug effects*
  • Schwann Cells / physiology
  • Sciatic Nerve / drug effects*
  • Sciatic Nerve / metabolism
  • Sciatic Nerve / physiology
  • Tissue Engineering / methods*
  • Tissue Scaffolds / chemistry

Substances

  • Glucosides
  • Nerve Growth Factors
  • Neuroprotective Agents
  • Phenols
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polyglycolic Acid
  • Lactic Acid
  • rhodioloside