Epigallocatechin-3-gallate attenuates acrylamide-induced apoptosis and astrogliosis in rat cerebral cortex

Toxicol Mech Methods. 2017 May;27(4):298-306. doi: 10.1080/15376516.2017.1279251. Epub 2017 Jan 22.


The potent neurotoxic agent acrylamide (ACR) is formed during Maillard reaction in food processing. Epigallocatechin-3-gallate (EGCG), a major bioactive component of green tea, is an antioxidant, but its effects on ACR-induced neurotoxicity are unclear. Here, we investigated the neuroprotective effects of EGCG against ACR-induced apoptosis and astrogliosis in the cerebral cortex. Rats were pretreated with EGCG for 4 d and then co-administered ACR for 14 d. Immunohistochemical analysis of glial fibrillary acidic protein and 8-hydroxy-2'-deoxyguanosine indicated that EGCG attenuated astrogliosis and DNA damage in ACR-treated rats. Analysis of DNA fragmentation and protein expression of Bax, Bcl-2, caspase 3, and cytochrome c revealed that EGCG inhibited ACR-induced apoptosis. Furthermore, EGCG inhibited oxidative stress by enhancing the activity of antioxidant enzymes and glutathione levels and reducing the formation of reactive oxygen species and lipid peroxidation. Taken together, our data demonstrate that EGCG inhibits ACR-induced apoptosis and astrogliosis in the cerebral cortex.

Keywords: Acrylamide; apoptosis; astrogliosis; epigallocatechin-3-gallate; oxidative stress.

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Acrylamide / toxicity*
  • Animals
  • Apoptosis / drug effects*
  • Astrocytes / drug effects*
  • Astrocytes / metabolism
  • Astrocytes / pathology
  • Catechin / analogs & derivatives*
  • Catechin / pharmacology
  • Cerebral Cortex / drug effects*
  • Cerebral Cortex / metabolism
  • Cerebral Cortex / pathology
  • DNA Damage
  • Deoxyguanosine / analogs & derivatives
  • Deoxyguanosine / metabolism
  • Dose-Response Relationship, Drug
  • Glial Fibrillary Acidic Protein / metabolism
  • Gliosis / prevention & control*
  • Lipid Peroxidation / drug effects
  • Male
  • Neuroprotective Agents / pharmacology*
  • Oxidative Stress / drug effects
  • Rats, Sprague-Dawley


  • Glial Fibrillary Acidic Protein
  • Neuroprotective Agents
  • Acrylamide
  • 8-Hydroxy-2'-Deoxyguanosine
  • Catechin
  • epigallocatechin gallate
  • Deoxyguanosine