Corilagin Ameliorates the Extreme Inflammatory Status in Sepsis Through TLR4 Signaling Pathways

BMC Complement Altern Med. 2017 Jan 5;17(1):18. doi: 10.1186/s12906-016-1533-y.

Abstract

Background: Sepsis is one of the serious disorders in clinical practice. Recent studies found toll-like receptors 4 (TLR4) played an important role in sepsis. In this study, we tried to find the influence of Corilagin on TLR4 signal pathways in vitro and in vivo.

Methods: The cellular and animal models of sepsis were established by LPS and then interfered with Corilagin. Real-time PCR and western blot were employed to detect the mRNA and protein expressions of TLR4, MyD88, TRIF and TRAF6. ELISA was used to determine the IL-6 and IL-1β levels in supernatant and serum.

Results: The survival rate was improved in the LPS + Corilagin group, and the mRNA and protein expressions of TLR4, MyD88, TRIF and TRAF6 were significantly decreased than that in the LPS group both in cellular and animal models (P < 0.01). The pro-inflammatory cytokines IL-6 and IL-1β were greatly decreased in the LPS + Corilagin group both in supernatant and serum (P < 0.01).

Conclusions: Corilagin exerts the anti-inflammatory effects by down-regulating the TLR4 signaling molecules to ameliorate the extreme inflammatory status in sepsis.

Keywords: Corilagin; Epsis; Pro-inflammatory cytokines; Signal pathway; TLR4.

MeSH terms

  • Animals
  • Glucosides / administration & dosage*
  • Humans
  • Hydrolyzable Tannins / administration & dosage*
  • Interleukin-1beta / genetics
  • Interleukin-1beta / immunology
  • Interleukin-6 / genetics
  • Interleukin-6 / immunology
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Myeloid Differentiation Factor 88 / genetics
  • Myeloid Differentiation Factor 88 / immunology
  • RAW 264.7 Cells
  • Sepsis / drug therapy*
  • Sepsis / genetics
  • Sepsis / immunology
  • Signal Transduction / drug effects
  • Toll-Like Receptor 4 / genetics
  • Toll-Like Receptor 4 / immunology*

Substances

  • Glucosides
  • Hydrolyzable Tannins
  • Interleukin-1beta
  • Interleukin-6
  • Myeloid Differentiation Factor 88
  • Tlr4 protein, mouse
  • Toll-Like Receptor 4
  • corilagin