ORCAN-a web-based meta-server for real-time detection and functional annotation of orthologs

Bioinformatics. 2017 Apr 15;33(8):1224-1226. doi: 10.1093/bioinformatics/btw825.


Summary: ORCAN (ORtholog sCANner) is a web-based meta-server for one-click evolutionary and functional annotation of protein sequences. The server combines information from the most popular orthology-prediction resources, including four tools and four online databases. Functional annotation utilizes five additional comparisons between the query and identified homologs, including: sequence similarity, protein domain architectures, functional motifs, Gene Ontology term assignments and a list of associated articles. Furthermore, the server uses a plurality-based rating system to evaluate the orthology relationships and to rank the reference proteins by their evolutionary and functional relevance to the query. Using a dataset of ∼1 million true yeast orthologs as a sample reference set, we show that combining multiple orthology-prediction tools in ORCAN increases the sensitivity and precision by 1-2 percent points.

Availability and implementation: The service is available for free at http://www.combio.pl/orcan/ .

Contact: wmk@amu.edu.pl.

Supplementary information: Supplementary data are available at Bioinformatics online.

MeSH terms

  • Computational Biology / methods*
  • Gene Ontology
  • Internet*
  • Protein Domains
  • Sequence Analysis, Protein / methods*
  • Sequence Homology, Amino Acid*
  • Software*
  • Yeasts / genetics
  • Yeasts / metabolism