Identification and Quantitation of Potent Odorants in Spearmint Oils

J Agric Food Chem. 2018 Mar 14;66(10):2414-2421. doi: 10.1021/acs.jafc.6b04852. Epub 2017 Jan 17.


Potent odorants in Native spearmint, Scotch spearmint, and Macho mint oils were determined by the combined use of gas chromatography-olfactometry (GCO), gas chromatography-mass spectrometry (GC-MS), and aroma extract dilution analysis (AEDA). Of the 85 odorants detected, ( R)-(-)-carvone was the most potent odorant in all three spearmint oils. Additional predominant odorants in all spearmint oils included eugenol, ethyl ( S)-(+)-2-methylbutanoate, ( E)-β-damascenone, and (3 E,5 Z)-1,3,5-undecatriene. Forty-six compounds were quantitated using various methods, including 19 by gas chromatography with flame ionization detection (GC-FID), 20 by stable isotope dilution analysis (SIDA), and 14 by GCO dilution analysis. Concentrations were used to calculate the odor activity values (OAVs) for predominant odorants in the oils. Among the compounds quantitated, those with the highest OAVs were ( R)-(-)-carvone, 1,8-cineole, ( E, Z)-2,6-nonadienal, ( E)-β-damascenone, and (3 E,5 Z)-1,3,5-undecatriene.

Keywords: aroma extract dilution analysis (AEDA); carvone; flavor chemistry; gas chromatography−olfactometry (GCO); mint; odor activity value (OAV); spearmint; stable isotope dilution analysis (SIDA).

MeSH terms

  • Eugenol / chemistry
  • Gas Chromatography-Mass Spectrometry
  • Mentha spicata / chemistry*
  • Norisoprenoids / chemistry
  • Odorants / analysis*
  • Plant Oils / chemistry*
  • Volatile Organic Compounds / chemistry*


  • Norisoprenoids
  • Plant Oils
  • Volatile Organic Compounds
  • Eugenol
  • beta-damascenone