Daboialectin, a C-type lectin from Russell's viper venom induces cytoskeletal damage and apoptosis in human lung cancer cells in vitro

Abstract

'Daboialectin', a low molecular weight C-type lectin (18.5 kDa) isolated from Russell's viper venom showed cytotoxic effects on human broncho-alveolar carcinoma derived (A549) cell lines. Daboialectin induced inhibition of A549 cell growth was time and concentration dependent with severe morphological changes by altering the functions of small GTPases such as Rac, Rho and cdc42. ROS induced DNA damage may result in apoptosis by inducing disruption of membrane integrity, blebbing and nuclear disintegration by activating caspases. Our results indicate that Daboialectin, a snake c type lectin (Snaclec) isolated from RVV alters morphology of A549 cells via regulation of cytoskeleton through RHO-GTPases. On other hand, the HSP70 and some other anti-apoptotic proteins required for the survival of cancer cells was found to be down-regulated in presence of Daboialectin. Daboialectin was also found to be inhibitory to anti-adhesive and anti-invasive to A549 cells in vitro. Daboialectin is the first Snaclec reported to induce cytoskeletal changes through regulation of RHO-GTPases and blocking anti-apoptotic pathway for a cancer cell line.

Keywords: A549 cells; Apoptosis; Cytotoxicity; Daboialectin; RHO-GTPases; Russell's viper venom.