A new method of genotyping MDX 4CV mice by PCR-RFLP analysis

Muscle Nerve. 2017 Sep;56(3):522-524. doi: 10.1002/mus.25566. Epub 2017 Mar 21.

Abstract

Introduction: The mdx4cv mouse is a common model to study Duchenne muscular dystrophy. The most used methodology to identify the genotype of these mice is Sanger DNA sequencing.

Methods: Here, we provide a simple, cost-effective alternative approach to identify the wild-type, heterozygous, or homozygous/hemizygous genotypes of these mice, using commonly available laboratory equipment and reagents.

Results: Our technique exploits a restriction fragment length polymorphism that is generated by the point mutation found in exon 53 of mdx4cv mice.

Conclusions: This technique can benefit laboratories that require complex breeding strategies involving mdx4cv mice. Muscle Nerve 56: 522-524, 2017.

Keywords: DMD; Duchenne muscular dystrophy; PCR-RFLP; genotyping; mdx; mdx4cv; restriction fragment length polymorphism.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Female
  • Genotype*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred mdx
  • Muscular Dystrophy, Duchenne / genetics*
  • Polymorphism, Restriction Fragment Length / genetics*
  • Real-Time Polymerase Chain Reaction / methods*