Introduction: The mdx4cv mouse is a common model to study Duchenne muscular dystrophy. The most used methodology to identify the genotype of these mice is Sanger DNA sequencing.
Methods: Here, we provide a simple, cost-effective alternative approach to identify the wild-type, heterozygous, or homozygous/hemizygous genotypes of these mice, using commonly available laboratory equipment and reagents.
Results: Our technique exploits a restriction fragment length polymorphism that is generated by the point mutation found in exon 53 of mdx4cv mice.
Conclusions: This technique can benefit laboratories that require complex breeding strategies involving mdx4cv mice. Muscle Nerve 56: 522-524, 2017.
Keywords: DMD; Duchenne muscular dystrophy; PCR-RFLP; genotyping; mdx; mdx4cv; restriction fragment length polymorphism.
© 2017 Wiley Periodicals, Inc.