Diversity in VP3, NSP3, and NSP4 of rotavirus B detected from Japanese cattle

Infect Genet Evol. 2017 Apr;49:97-103. doi: 10.1016/j.meegid.2017.01.003. Epub 2017 Jan 4.

Abstract

Bovine rotavirus B (RVB) is an etiological agent of diarrhea mostly in adult cattle. Currently, a few sequences of viral protein (VP)1, 2, 4, 6, and 7 and nonstructural protein (NSP)1, 2, and 5 of bovine RVB are available in the DDBJ/EMBL/GenBank databases, and none have been reported for VP3, NSP3, and NSP4. In order to fill this gap in the genetic characterization of bovine RVB strains, we used a metagenomics approach and sequenced and analyzed the complete coding sequences (CDS) of VP3, NSP3, and NSP4 genes, as well as the partial or complete CDS of other genes of RVBs detected from Japanese cattle. VP3, NSP3, and NSP4 of bovine RVBs shared low nucleotide sequence identities (63.3-64.9% for VP3, 65.9-68.2% for NSP3, and 52.6-56.2% for NSP4) with those of murine, human, and porcine RVBs, suggesting that bovine RVBs belong to a novel genotype. Furthermore, significantly low amino acid sequence identities were observed for NSP4 (36.1-39.3%) between bovine RVBs and the RVBs of other species. In contrast, hydrophobic plot analysis of NSP4 revealed profiles similar to those of RVBs of other species and rotavirus A (RVA) strains. Phylogenetic analyses of all gene segments revealed that bovine RVB strains formed a cluster that branched distantly from other RVBs. These results suggest that bovine RVBs have evolved independently from other RVBs but in a similar manner to other rotaviruses. These findings provide insights into the evolution and diversity of RVB strains.

Keywords: Bovine rotavirus B; Genetic characterization; Japan; Metagenomics approach; Novel genotype.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Evolution
  • Capsid Proteins / genetics*
  • Cattle
  • Cluster Analysis
  • Databases, Genetic
  • Feces / virology
  • Genetic Variation
  • Genome, Viral*
  • Genotype
  • Glycoproteins / genetics*
  • Japan / epidemiology
  • Phylogeny*
  • Rotavirus / classification
  • Rotavirus / genetics*
  • Rotavirus Infections / epidemiology
  • Rotavirus Infections / virology
  • Toxins, Biological / genetics*
  • Viral Nonstructural Proteins / genetics*

Substances

  • Capsid Proteins
  • Glycoproteins
  • NS28 protein, rotavirus
  • NSP3 protein, Rotavirus
  • Toxins, Biological
  • VP3 protein, Rotavirus
  • Viral Nonstructural Proteins