Analysis of protein dynamics during cytokinesis in budding yeast

Methods Cell Biol. 2017;137:25-45. doi: 10.1016/bs.mcb.2016.04.002. Epub 2016 Jun 11.

Abstract

Cytokinesis is essential for development and survival of all organisms by increasing cell number and diversity. It is a highly regulated process that requires spatiotemporal coordination of hundreds of proteins functioning in the assembly, constriction, and disassembly of a contractile actomyosin ring, targeted vesicle fusion, and localized extracellular matrix remodeling. Cytokinesis has been studied in multiple systems with a wide range of technologies to learn the common principles. In this chapter, we describe the analysis of protein dynamics during cytokinesis in the budding yeast Saccharomyces cerevisiae by several live-cell imaging methods. This, in combination with the power of yeast genetics, has yielded novel insights into the mechanism of cytokinesis. Similar approaches are increasingly used to study this fundamental process in more complex systems.

Keywords: Cytokinesis; Myosin; Saccharomyces cerevisiae; Septin; Septum formation.

MeSH terms

  • Actomyosin / chemistry
  • Actomyosin / metabolism
  • Cytokinesis / genetics*
  • Extracellular Matrix / metabolism
  • Molecular Biology / methods*
  • Molecular Imaging / methods*
  • Myosins / chemistry
  • Myosins / metabolism
  • Saccharomyces cerevisiae / chemistry
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism
  • Septins / biosynthesis
  • Septins / metabolism

Substances

  • Saccharomyces cerevisiae Proteins
  • Actomyosin
  • Septins
  • Myosins