Human embryonic myosin heavy chain cDNA. Interspecies sequence conservation of the myosin rod, chromosomal locus and isoform specific transcription of the gene

FEBS Lett. 1989 Oct 9;256(1-2):21-8. doi: 10.1016/0014-5793(89)81710-7.


A 3.6 kilobase cDNA clone coding for the human embryonic myosin heavy chain has been isolated and characterized from an expression library prepared from human fetal skeletal muscle. The derived amino acid sequence for the entire rod part of myosin shows 97% sequence homology between human and rat and a striking interspecies sequence conservation among the charged amino acid residues. The single copy gene is localized to human chromosome 17 and its expression in fetal skeletal muscle is developmentally regulated. The sequence information permits the design of isoform-specific probes for studies on the structure of the gene and its role in normal and defective human myogenesis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Southern
  • Chromosome Mapping*
  • Chromosomes, Human, Pair 17*
  • DNA / isolation & purification*
  • Fetus / metabolism*
  • Gene Expression
  • Genes
  • Humans
  • Molecular Sequence Data
  • Muscle Development
  • Muscles / analysis
  • Muscles / metabolism*
  • Myosin Subfragments / analysis
  • Myosin Subfragments / genetics*
  • Rats
  • Sequence Homology, Nucleic Acid
  • Species Specificity
  • Transcription, Genetic


  • Myosin Subfragments
  • DNA

Associated data

  • GENBANK/X13100