Ebola Vaccination Using a DNA Vaccine Coated on PLGA-PLL/γPGA Nanoparticles Administered Using a Microneedle Patch

Adv Healthc Mater. 2017 Jan;6(1). doi: 10.1002/adhm.201600750. Epub 2016 Nov 7.

Abstract

Ebola DNA vaccine is incorporated into PLGA-PLL/γPGA nanoparticles and administered to skin using a microneedle (MN) patch. The nanoparticle delivery system increases vaccine thermostability and immunogenicity compared to free vaccine. Vaccination by MN patch produces stronger immune responses than intramuscular administration.

Keywords: Ebola DNA vaccine; cationic nanoparticles; indradermal vaccine delivery; microneedle patch; minimally invasive skin vaccination.

MeSH terms

  • Animals
  • Ebola Vaccines* / chemistry
  • Ebola Vaccines* / pharmacology
  • HeLa Cells
  • Humans
  • Immunogenicity, Vaccine
  • Lactic Acid* / chemistry
  • Lactic Acid* / pharmacology
  • Mice
  • Nanoparticles* / chemistry
  • Nanoparticles* / therapeutic use
  • Needles
  • Polyglutamic Acid* / chemistry
  • Polyglutamic Acid* / pharmacology
  • Polyglycolic Acid* / chemistry
  • Polyglycolic Acid* / pharmacology
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polylysine* / chemistry
  • Polylysine* / pharmacology
  • Vaccines, DNA* / chemistry
  • Vaccines, DNA* / pharmacology

Substances

  • Ebola Vaccines
  • Vaccines, DNA
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polylysine
  • Polyglutamic Acid
  • Polyglycolic Acid
  • Lactic Acid