Synergism between interleukin-1 beta and tumor necrosis factor-alpha in production by 3T3 cells of a chemotactic factor for rat polymorphonuclear leukocytes

Inflammation. 1989 Oct;13(5):553-9. doi: 10.1007/BF00916761.

Abstract

Recombinant human interleukin-1 beta (IL-1) and tumor necrosis factor-alpha (TNF) synergistically stimulated BALB/c 3T3 cells to produce a chemotactic factor for rat polymorphonuclear leukocytes (PMNs), whereas an addition of 10(-11)-10(-8) M IL-1 or TNF alone to the cell culture resulted in a slight increase in the production of chemotactic factor. The partially purified factor was not a chemokinetic but chemotactic factor for PMNs when analyzed by checkerboard analysis. The partially purified factor was trypsin sensitive and heat stable; its isoelectric point was 8.5-10, and its molecular weight was about 10 kDa as estimated by gel filtration. These results suggest that fibroblasts may participate in PMN migration to the inflammatory site where both IL-1 and TNF are released by activated inflammatory cells, including macrophages.

MeSH terms

  • Animals
  • Cells, Cultured
  • Chemotactic Factors / biosynthesis*
  • Chemotactic Factors / isolation & purification
  • Chemotactic Factors / pharmacology
  • Chemotaxis, Leukocyte / drug effects
  • Drug Synergism
  • Fibroblasts / drug effects*
  • Fibroblasts / metabolism
  • Humans
  • Interleukin-1 / pharmacology*
  • Mice
  • Mice, Inbred BALB C
  • Neutrophils / drug effects*
  • Rats
  • Stimulation, Chemical
  • Tumor Necrosis Factor-alpha / pharmacology*

Substances

  • Chemotactic Factors
  • Interleukin-1
  • Tumor Necrosis Factor-alpha