Comparison of single nucleotide polymorphisms [SNP] at TNF-α promoter region with TNF receptor 2 (TNFR2) in susceptibility to pulmonary tuberculosis; using PCR-RFLP technique

Ehsan Ghamari; Poopak Farnia; Shima Saif; Mehran Marashian; Jaladein Ghanavi; Parissa Farnia; Ali Akbar Velayati

Comparison of single nucleotide polymorphisms [SNP] at TNF-α promoter region with TNF receptor 2 (TNFR2) in susceptibility to pulmonary tuberculosis; using PCR-RFLP technique

Am J Clin Exp Immunol. 2016 Nov 30;5(4):55-61. eCollection 2016.

Authors

Ehsan Ghamari¹, Poopak Farnia², Shima Saif¹, Mehran Marashian¹, Jaladein Ghanavi¹, Parissa Farnia¹, Ali Akbar Velayati¹

Affiliations

¹ Mycobacteriology Research Centre (MRC), National Research Institute of Tuberculosis and Lung Disease (NRITLD), Shahid Beheshti University of Medical Sciences Tehran, Iran.
² Mycobacteriology Research Centre (MRC), National Research Institute of Tuberculosis and Lung Disease (NRITLD), Shahid Beheshti University of Medical SciencesTehran, Iran; Department of Biotechnology, Advanced Technologies in Medicine, Shahid Beheshti University of Medical SciencesTehran, Iran.

PMID: 28078191
PMCID: PMC5218852

Abstract

Since apoptosis and survival of the immune cells are crucially important in prevention or predisposition of individual from/to infections, especially in intracellular ones, the current study was performed to assess the correlation of host genetic polymorphisms with susceptibility to TB. For this reason, we investigated the difference of the single nucleotide polymorphisms [SNPs] in tumor necrosis factors [TNF-α] genes at (-238, -308, -857 and -863 position) and tumor necrosis factors receptors two [TNFR2] at (T 587 G position) between patients [n=151] and control [n=83]. The genotyping was studied by using PCR-RFLP which had high sensitivity in detecting compared with other techniques. The results showed a strong correlation between two polymorphisms in different loci of TNF-α gene including TNF-α T-857 C and A 238 G. But no association were found in TNFR2 genes with susceptibility to TB. And we found no correlation between TNFR2 and TNF-α gene polymorphisms. Therefore, the TNF-α T 857 C and A 238 G SNPs could be promising marker for identifying risk populations.

Keywords: Iran; PCR-RFLP; Pulmonary tuberculosis; TNF alpha; TNFR2; susceptibility.