Stimulation by transforming growth factor-beta of epidermal growth factor-dependent growth of aged human fibro-blasts: recovery of high affinity EGE receptors and growth stimulation by EGF

In Vitro Cell Dev Biol. 1989 Oct;25(10):965-70. doi: 10.1007/BF02624011.


The stimulatory effects of transforming growth factor beta (TGF-beta) on epidermal growth factor (EGF)-dependent growth of adult and newborn human fibroblasts were investigated. EGF-stimulated growth in low serum of dermal fibroblasts from a 41 year-old adult (HSF-41) was less than half that of newborn foreskin fibroblasts (HFF). The EGF-stimulated growth of HFF after 55 population doublings (HFF-55) was similarly reduced. The decreased growth response to EGF of fibroblasts aged in vivo and in vitro appeared to result principally from a decreased sensitivity to EGF due to a decreased number and affinity of high affinity EGF receptors (H-EGFR). Pre-incubation of HSF-41 and HFF-55 with 25 pM TGF-beta enhanced the growth responses of these cells to EGF and increased the levels of high affinity EGF-binding by these cells. Thus, the stimulation by TGF-beta of EGF-dependent growth of human fibroblasts aged in vivo or in vitro is mediated by increased levels of high affinity EGF binding.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Biopsy
  • Cell Division / drug effects
  • Cells, Cultured
  • Epidermal Growth Factor / metabolism
  • Epidermal Growth Factor / pharmacology*
  • ErbB Receptors / analysis
  • ErbB Receptors / metabolism
  • Fibroblasts / cytology*
  • Fibroblasts / drug effects
  • Fibroblasts / ultrastructure
  • Humans
  • Infant, Newborn
  • Male
  • Skin / cytology
  • Skin / ultrastructure
  • Transforming Growth Factors / pharmacology*


  • Epidermal Growth Factor
  • Transforming Growth Factors
  • ErbB Receptors