Hydrogen-rich saline attenuates hippocampus endoplasmic reticulum stress after cardiac arrest in rats

Neurosci Lett. 2017 Feb 15;640:29-36. doi: 10.1016/j.neulet.2017.01.020. Epub 2017 Jan 10.


Background: Hydrogen-rich saline can selectively scavenge reactive oxygen species (ROS) and protect brain against ischemia reperfusion (I/R) injury. Endoplasmic reticulum stress (ERS) has been implicated in the pathological process of cerebral ischemia. However, very little is known about the role of hydrogen-rich saline in mediating pathophysiological reactions to ERS after I/R injury caused by cardiac arrest.

Methods: The rats were randomly divided into three groups, sham group (n=30), ischemia/reperfusion group (n=40) and hydrogen-rich saline group (n=40). The rats in experimental groups were subjected to 4min of cardiac arrest and followed by resuscitation. Then they were randomized to receive 5ml/kg of either hydrogen-rich saline or normal saline.

Results: Hydrogen-rich saline significantly improves survival rate and neurological function. The beneficial effects of hydrogen-rich saline were associated with decreased levels of oxidative products, as well as the increased levels of antioxidant enzymes. Furthermore, the protective effects of hydrogen-rich saline were accompanied by the increased activity of glucose-regulated protein 78 (GRP78), the decreased activity of cysteinyl aspartate specific proteinase-12 (caspase-12) and C/EBP homologous protein (CHOP).

Conclusions: Hydrogen-rich saline attenuates brain I/R injury may through inhibiting hippocampus ERS after cardiac arrest in rats.

Keywords: Apoptosis; CPR; Endoplasmic reticulum stress; Hydrogen; Oxidative stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Antioxidants / therapeutic use
  • Endoplasmic Reticulum / drug effects
  • Endoplasmic Reticulum / ultrastructure
  • Endoplasmic Reticulum Stress / drug effects*
  • Heart Arrest / pathology
  • Heart Arrest / physiopathology
  • Heart Arrest / therapy*
  • Hippocampus / metabolism*
  • Hippocampus / pathology
  • Hydrogen / pharmacology*
  • Hydrogen / therapeutic use
  • Male
  • Oxidative Stress / drug effects
  • Random Allocation
  • Rats, Sprague-Dawley
  • Sodium Chloride / pharmacology*
  • Sodium Chloride / therapeutic use
  • Survival Rate


  • Antioxidants
  • Sodium Chloride
  • Hydrogen