ATP Binding Cassette Transporter ABCA7 Regulates NKT Cell Development and Function by Controlling CD1d Expression and Lipid Raft Content

Sci Rep. 2017 Jan 16;7:40273. doi: 10.1038/srep40273.

Abstract

ABCA7 is an ABC transporter expressed on the plasma membrane, and actively exports phospholipid complexes from the cytoplasmic to the exocytoplasmic leaflet of membranes. Invariant NKT (iNKT) cells are a subpopulation of T lymphocytes that recognize glycolipid antigens in the context of CD1d-mediated antigen presentation. In this study, we demonstrate that ABCA7 regulates the development of NKT cells in a cell-extrinsic manner. We found that in Abca7-/- mice there is reduced expression of CD1d accompanied by an alteration in lipid raft content on the plasma membrane of thymocytes and antigen presenting cells. Together, these alterations caused by absence of ABCA7 negatively affect NKT cell development and function.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • ATP-Binding Cassette Transporters / genetics
  • ATP-Binding Cassette Transporters / immunology
  • ATP-Binding Cassette Transporters / metabolism*
  • Animals
  • Antigens, CD1d / metabolism*
  • Female
  • Male
  • Membrane Microdomains / immunology
  • Membrane Microdomains / metabolism*
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Natural Killer T-Cells / immunology
  • Natural Killer T-Cells / metabolism*

Substances

  • ATP-Binding Cassette Transporters
  • Abca7 protein, mouse
  • Antigens, CD1d
  • CD1d antigen, mouse