Isolation and characterization of Rhizobium sp. strain YS-1r that degrades lignin in plant biomass

J Appl Microbiol. 2017 Apr;122(4):940-952. doi: 10.1111/jam.13401. Epub 2017 Feb 27.

Abstract

Aims: The aim of this work was to isolate novel lignin-degrading organisms.

Methods and results: Several pure cultures of bacteria that degrade lignin were isolated from bacterial consortia developed from decaying biomass. Among the isolates, Rhizobium sp. strain YS-1r (closest relative of Rhizobium petrolearium strain SL-1) was explored for its lignin-degrading ability. Microcosm studies showed that strain YS-1r was able to degrade a variety of lignin monomers, dimers and also native lignin in switchgrass and alfalfa. The isolate demonstrated lignin peroxidase (LiP) activity when grown on alkali lignin, p-anisoin, switchgrass or alfalfa, and only negligible activity was measured in glucose-grown cells suggesting inducible nature of the LiP activity. Analysis of the strain YS-1r genome revealed the presence of a variety of genes that code for various lignin-oxidizing, H2 O2 -producing as well as polysaccharide-hydrolysing enzymes.

Conclusions: This study shows both the genomic and physiological capability of bacteria in the genus Rhizobium to metabolize lignin and lignin-like compounds. This is the first detailed report on the lignocellulose-degrading ability of a Rhizobium species and thus this study expands the role of alpha-proteobacteria in the degradation of lignin.

Significance and impact of the study: The organism's ability to degrade lignin is significant since Rhizobia are widespread in soil, water and plant rhizospheres and some fix atmospheric nitrogen and also have the ability to degrade aromatic hydrocarbons.

Keywords: Alfalfa; Rhizobium sp.; lignin biodegradation; lignin-oxidizing genes; switchgrass.

MeSH terms

  • Biomass
  • Lignin / metabolism*
  • Medicago sativa / metabolism
  • Panicum / metabolism
  • Rhizobium / genetics
  • Rhizobium / isolation & purification
  • Rhizobium / metabolism*

Substances

  • Lignin

Associated data

  • GENBANK/JX257168
  • GENBANK/JX257172