Expression of MMP-2 and TIMP-1 during rapid maxillary expansion in rats

Jianwei Chen; Jing Zhou; Fan Li; Jianfeng Sun; Guifeng Li; Shujuan Zou; Qingsong Ye

doi:10.1016/j.archoralbio.2017.01.002

Expression of MMP-2 and TIMP-1 during rapid maxillary expansion in rats

Arch Oral Biol. 2017 Apr:76:30-35. doi: 10.1016/j.archoralbio.2017.01.002. Epub 2017 Jan 4.

Authors

Jianwei Chen¹, Jing Zhou², Fan Li³, Jianfeng Sun⁴, Guifeng Li⁵, Shujuan Zou⁶, Qingsong Ye⁷

Affiliations

¹ The State Key Laboratory of Oral Diseases and Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
² Stomatological Department, Yan'an Hospital of Kunming City, Kunming, China.
³ Department of Orthodontics and Institute of Dental Research, Beijing Stomatological Hospital and School of Stomatology, Capital Medical University, Beijing, China.
⁴ Orthodontic Department, Ningbo Dental Hospital, Ningbo City, China.
⁵ Department of Orthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing University, Nanjing, China.
⁶ The State Key Laboratory of Oral Diseases and Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China. Electronic address: shujuannzou@aliyun.com.
⁷ Department of Orthodontics, UQ Oral Health Centre, University of Queensland, Herston, QLD, Australia.

PMID: 28092867
DOI: 10.1016/j.archoralbio.2017.01.002

Abstract

Objective: The aim of this study was to investigate the expression of MMP-2 and TIMP-1 during midpalatal suture expansion in rats.

Design: 72 male Wistar rats were randomly divided into 2 groups: the experimental group and the control group. In the experimental group, opening loops were applied across the midpalatal suture with an initial force of 50g, whereas in the control group, rats were subjected to sham installation of opening loops without activation. On day 1, 4, 7 and 14, nine rats from each group were sacrificed, and the maxillae were dissected and prepared for Immunohistochemistry (IHC) and RT- PCR examination of MMP-2 and TIMP-1 expression.

Results: The results of IHC and Real Time PCR revealed that both protein and mRNA expression of MMP-2 and TIMP-1 were significantly increased after midpalatal expansion, and the ratio of MMP-2/TIMP-1 was also significantly enhanced.

Conclusions: The data suggested that MMP-2 and TIMP-1 might play an important role during the mid-palatal suture remodeling process of maxillary expansion.

Keywords: Bone remodeling; Matrix metalloproteinase; Midpalatal suture; Tensile force; Tissue inhibitors of metalloproteinase.

MeSH terms

Animals
Bone Remodeling
Male
Matrix Metalloproteinase 2 / metabolism*
Maxilla / metabolism*
Palatal Expansion Technique*
Rats
Rats, Wistar
Real-Time Polymerase Chain Reaction
Tensile Strength
Tissue Inhibitor of Metalloproteinase-1 / metabolism*

Substances

Tissue Inhibitor of Metalloproteinase-1
Matrix Metalloproteinase 2