Targeted DNA sequencing and in situ mutation analysis using mobile phone microscopy

Nat Commun. 2017 Jan 17;8:13913. doi: 10.1038/ncomms13913.

Abstract

Molecular diagnostics is typically outsourced to well-equipped centralized laboratories, often far from the patient. We developed molecular assays and portable optical imaging designs that permit on-site diagnostics with a cost-effective mobile-phone-based multimodal microscope. We demonstrate that targeted next-generation DNA sequencing reactions and in situ point mutation detection assays in preserved tumour samples can be imaged and analysed using mobile phone microscopy, achieving a new milestone for tele-medicine technologies.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Phone / statistics & numerical data*
  • DNA / genetics*
  • Humans
  • Microscopy
  • Mutation
  • Neoplasms / diagnosis*
  • Neoplasms / genetics
  • Pathology, Molecular / instrumentation
  • Pathology, Molecular / methods*
  • Proto-Oncogene Proteins p21(ras) / genetics
  • Sequence Analysis, DNA / instrumentation
  • Sequence Analysis, DNA / methods*

Substances

  • KRAS protein, human
  • DNA
  • Proto-Oncogene Proteins p21(ras)