Sensitive radioimmunoassays for histamine and tele-methylhistamine in the brain

J Neurochem. 1989 Dec;53(6):1724-30. doi: 10.1111/j.1471-4159.1989.tb09237.x.


Serum albumin conjugates of histamine or tele-methylhistamine, a major catabolite, were prepared using 1,4-benzoquinone as the coupling agent and used to raise polyclonal antibodies in rabbits. The same reagent was used to prepare the [125I]iodinated tracer and treat tissue extracts submitted to the radioimmunoassays. The IC50 values of prederivatized histamine and tele-methylhistamine in the radioimmunoassays were 0.3 nM and 0.5 nM, respectively, whereas nonderivatized histamine or tele-methylhistamine, histidine, a variety of histamine derivatives, amines, etc., had at least 1,000-fold higher IC50 values. Application of the radioimmunoassays to nonpurified extracts of rat brain allowed the quantification of the two amine immunoreactivities in samples corresponding to less than 1 mg of hypothalamus. The tissue immunoreactivity corresponded to authentic histamine or tele-methylhistamine, as shown by (a) the parallel 125I-tracer displacement curves, (b) the similar elution patterns from HPLC columns, (c) the regional levels of histamine and tele-methylhistamine in brain, similar to those obtained with other methods, and (d) the clearcut effects of treatments with inhibitors of L-histidine decarboxylase or monoamine oxidase. The two radioimmunoassays appear as simple and sensitive tools to evaluate steady-state levels and turnover rates of histamine and tele-methylhistamine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain Chemistry*
  • Cerebral Cortex / drug effects
  • Cerebral Cortex / metabolism
  • Chromatography, High Pressure Liquid
  • Cross Reactions
  • Histamine / analysis*
  • Histamine / metabolism
  • Histidine Decarboxylase / antagonists & inhibitors
  • Male
  • Methylhistamines / analysis*
  • Methylhistamines / metabolism
  • Methylhistidines / pharmacology
  • Organ Specificity
  • Pargyline / pharmacology
  • Piperidines / pharmacology
  • Radioimmunoassay / methods
  • Rats
  • Rats, Inbred Strains
  • Reference Values
  • Tritium


  • Methylhistamines
  • Methylhistidines
  • Piperidines
  • Tritium
  • alpha-fluoromethylhistidine
  • Histamine
  • Pargyline
  • Histidine Decarboxylase
  • thioperamide
  • tele-methylhistamine