The Power of CRISPR-Cas9-Induced Genome Editing to Speed Up Plant Breeding

Int J Genomics. 2016;2016:5078796. doi: 10.1155/2016/5078796. Epub 2016 Dec 20.


Genome editing with engineered nucleases enabling site-directed sequence modifications bears a great potential for advanced plant breeding and crop protection. Remarkably, the RNA-guided endonuclease technology (RGEN) based on the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) is an extremely powerful and easy tool that revolutionizes both basic research and plant breeding. Here, we review the major technical advances and recent applications of the CRISPR-Cas9 system for manipulation of model and crop plant genomes. We also discuss the future prospects of this technology in molecular plant breeding.

Publication types

  • Review