Ectopic expression of two AREB/ABF orthologs increases drought tolerance in cotton (Gossypium hirsutum)

Plant Cell Environ. 2018 May;41(5):898-907. doi: 10.1111/pce.12906. Epub 2017 Mar 30.

Abstract

Plants have evolved complex molecular, cellular and physiological mechanisms to respond to environmental stressors. Because of the inherent complexity of this response, genetic manipulation to substantially improve water deficit tolerance, particularly in agricultural crops, has been largely unsuccessful, as the improvements are frequently accompanied by slower growth and delayed reproduction. Here, we ectopically express two abiotic stress-responsive bZIP AREB/ABF transcription factor orthologs, Arabidopsis ABF3 and Gossypium hirsutum ABF2D, in G. hirsutum, to compare the effects of exogenous and endogenous AREB/ABF transgene overexpression on dehydration resilience. Our results show that ectopic expression of each of these orthologs increases dehydration resilience, although these increases are accompanied by slower growth. These phenotypic effects are proportional to the ectopic expression level in the GhABF2D transgenic plants, while the phenotypes of all of the AtABF3 transgenic plants are similar, largely independent of ectopic expression level, possibly indicating differential post-transcriptional regulation of these transgenes. Our results indicate that overexpression of exogenous and endogenous ABF homologs in G. hirsutum substantially increases drought resilience, primarily through stomatal regulation, negatively impacting transpiration and photosynthetic productivity.

Keywords: abiotic stress; abscisic acid; drought; transgenic plants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Basic-Leucine Zipper Transcription Factors / genetics
  • Basic-Leucine Zipper Transcription Factors / metabolism*
  • Crops, Agricultural
  • Droughts
  • Ectopic Gene Expression
  • Gossypium / genetics
  • Gossypium / physiology*
  • Phenotype
  • Photosynthesis
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Plant Stomata / genetics
  • Plant Stomata / physiology
  • Plant Transpiration / physiology
  • Plants, Genetically Modified
  • Stress, Physiological
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*

Substances

  • ABF3 protein, Arabidopsis
  • Arabidopsis Proteins
  • Basic-Leucine Zipper Transcription Factors
  • Plant Proteins
  • Transcription Factors