Preventive Effects of Drinking Hydrogen-Rich Water on Gingival Oxidative Stress and Alveolar Bone Resorption in Rats Fed a High-Fat Diet

Nutrients. 2017 Jan 13;9(1):64. doi: 10.3390/nu9010064.


Obesity induces gingival oxidative stress, which is involved in the progression of alveolar bone resorption. The antioxidant effect of hydrogen-rich water may attenuate gingival oxidative stress and prevent alveolar bone resorption in cases of obesity. We examined whether hydrogen-rich water could suppress gingival oxidative stress and alveolar bone resorption in obese rats fed a high-fat diet. Male Fischer 344 rats (n = 18) were divided into three groups of six rats each: a control group (fed a regular diet and drinking distilled water) and two experimental groups (fed a high-fat diet and drinking distilled water or hydrogen-rich water). The level of 8-hydroxydeoxyguanosine was determined to evaluate oxidative stress. The bone mineral density of the alveolar bone was analyzed by micro-computerized tomography. Obese rats, induced by a high-fat diet, showed a higher gingival level of 8-hydroxydeoxyguanosine and a lower level of alveolar bone density compared to the control group. Drinking hydrogen-rich water suppressed body weight gain, lowered gingival level of 8-hydroxydeoxyguanosine, and reduced alveolar bone resorption in rats on a high-fat diet. The results indicate that hydrogen-rich water could suppress gingival oxidative stress and alveolar bone resorption by limiting obesity.

Keywords: alveolar bone loss; animal disease model; hydrogen-rich water; obesity; oxidative stress.

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Alveolar Bone Loss / prevention & control*
  • Animals
  • Antioxidants / administration & dosage
  • Antioxidants / analysis
  • Body Weight
  • Cholesterol, HDL / blood
  • Cholesterol, LDL / blood
  • Deoxyguanosine / analogs & derivatives
  • Deoxyguanosine / blood
  • Diet, High-Fat / adverse effects*
  • Drinking Water / administration & dosage*
  • Drinking Water / analysis
  • Fanconi Anemia Complementation Group C Protein / genetics
  • Fanconi Anemia Complementation Group C Protein / metabolism
  • Gene Expression
  • Gingiva / drug effects*
  • Gingiva / metabolism
  • Hydrogen / administration & dosage*
  • Isocitrate Dehydrogenase / genetics
  • Isocitrate Dehydrogenase / metabolism
  • Male
  • Oxidative Stress / drug effects*
  • Rats
  • Rats, Inbred F344
  • Superoxide Dismutase / genetics
  • Superoxide Dismutase / metabolism
  • Triglycerides / blood


  • Antioxidants
  • Cholesterol, HDL
  • Cholesterol, LDL
  • Drinking Water
  • Fancc protein, rat
  • Fanconi Anemia Complementation Group C Protein
  • Triglycerides
  • Hydrogen
  • 8-Hydroxy-2'-Deoxyguanosine
  • Isocitrate Dehydrogenase
  • Sod3 protein, rat
  • Superoxide Dismutase
  • superoxide dismutase 2
  • Deoxyguanosine