Virome of US bovine calf serum

Biologicals. 2017 Mar:46:64-67. doi: 10.1016/j.biologicals.2016.12.009. Epub 2017 Jan 15.


Using viral metagenomics we analyzed four bovine serum pools assembled from 715 calves in the United States. Two parvoviruses, bovine parvovirus 2 (BPV2) and a previously uncharacterized parvovirus designated as bosavirus (BosaV), were detected in 3 and 4 pools respectively and their complete coding sequences generated. Based on NS1 protein identity, bosavirus qualifies as a member of a new species in the copiparvovirus genus. Also detected were low number of reads matching ungulate tetraparvovirus 2, bovine hepacivirus, and several papillomaviruses. This study further characterizes the diversity of viruses in calf serum with the potential to infect fetuses and through fetal bovine serum contaminate cell cultures.

Keywords: Bosavirus; Cell culture; Metagenomics; Parvovirus.

MeSH terms

  • Animals
  • Bocavirus / classification
  • Bocavirus / genetics
  • Capsid Proteins / classification
  • Capsid Proteins / genetics
  • Cattle / blood*
  • Cattle / virology*
  • Genome, Viral / genetics*
  • Geography
  • Metagenomics / methods*
  • Parvoviridae Infections / veterinary
  • Parvoviridae Infections / virology
  • Phylogeny
  • Sequence Analysis, DNA
  • Serum / virology
  • Species Specificity
  • United States
  • Viral Nonstructural Proteins / classification
  • Viral Nonstructural Proteins / genetics


  • Capsid Proteins
  • Viral Nonstructural Proteins