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Review
. 2017 Apr 1;312(4):F640-F646.
doi: 10.1152/ajprenal.00369.2016. Epub 2017 Jan 18.

Mononuclear Phagocyte Subpopulations in the Mouse Kidney

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Free PMC article
Review

Mononuclear Phagocyte Subpopulations in the Mouse Kidney

James F George et al. Am J Physiol Renal Physiol. .
Free PMC article

Abstract

Mononuclear phagocytes are the most common cells in the kidney associated with immunity and inflammation. Although the presence of these cells in the kidney has been known for decades, the study of mononuclear phagocytes in the context of kidney function and dysfunction is still at an early stage. The purpose of this review is to summarize the present knowledge regarding classification of these cells in the mouse kidney and to identify relevant questions that would further advance the field and potentially lead to new opportunities for treatment of acute kidney injury and other kidney diseases.

Keywords: acute kidney injury; inflammation; ischemia-reperfusion; macrophages; renal fibrosis.

Figures

Fig. 1.
Fig. 1.
A schematic comparison of 3 schemes used to differentiate subpopulations of renal mononuclear phagocytes. Scheme A has been reproduced by a number of laboratories and shows populations consistent with embryological and fate-mapping studies, indicating that at least a portion of POP1 cells are derived from the embryonic yolk sac. Scheme B provides differentiation similar to that shown in Scheme A (pink and blue populations) and also indicates subpopulations containing CD103+ dendritic cells (DCs) (brown population). Because of a greater reliance on variations in fluorescence intensity, it is, however, critically dependent on the choice of fluorochromes, antibody clones, and cytometer settings. Therefore, it is more difficult to reproduce. Both Scheme A and Scheme B use similar preliminary gating based on forward and side scatter and selection-based CD45 and viability staining. Scheme C is additionally gated on MHCII+ cells. It provides excellent definition of DCs and allows differentiation of CD103+ DC with additional gating of CD11c+F4/80- cells (rMP3 and rMP4) based on CD103 and CD11b.

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