Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Dec;12(6):5235-5239.
doi: 10.3892/ol.2016.5359. Epub 2016 Nov 7.

Potential Role of Melastatin-Related Transient Receptor Potential Cation Channel Subfamily M Gene Expression in the Pathogenesis of Urinary Bladder Cancer

Affiliations
Free PMC article

Potential Role of Melastatin-Related Transient Receptor Potential Cation Channel Subfamily M Gene Expression in the Pathogenesis of Urinary Bladder Cancer

Gülay Güleç Ceylan et al. Oncol Lett. .
Free PMC article

Abstract

Urinary bladder cancer is one of the most common malignancies of the urinary tract. Ion channels and calcium homeostasis are involved in almost all basic cellular mechanisms. The transient receptor potential cation channel subfamily M (TRPM) takes its name from the melastatin protein, which is classified as potential tumor suppressor. To the best of our knowledge, there have been no previous studies in the literature investigating the role of these ion channels in bladder cancer. The present study aimed to determine whether bladder cancer is associated with mRNA expression levels of TRPM ion channel genes, and whether there is the potential to conduct further studies to establish novel treatment modalities. The present study included a total of 47 subjects, of whom 40 were bladder cancer patients and 7 were controls. Following the histopathological evaluation for bladder carcinoma, the mRNA and protein expression of TRPM were examined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and immunohistochemistry in tumor and normal tissues, in order to determine whether there is a difference in the expression of these channels in tumor and normal tissues. Immunoreactivity for TRPM2, TRPM4, TRPM7 and TRPM8 was observed in epithelial bladder cells in the two groups. RT-qPCR revealed a significant increase in TRPM7 expression in bladder cancer tissue compared to the controls (healthy bladder tissue), whereas no differences in TRPM2 or TRPM4 expression levels were observed. There were significant reductions in the expression levels of TRPM5 and TRPM8 in bladder cancer tissues. In the present study, the effects of TRP ion channels on the formation of bladder cancer was investigated. This study is instructive for TRPM2, TRPM4, TRPM5, TRPM7 and TRPM8 and their therapeutic role in bladder cancer. The results support the fact that these gens can be novel targets and can also be tested for during the treatment of bladder cancer.

Keywords: bladder cancer; expression; gene; immunohistochemistry; melastatin; quantitative polymerase chain reaction; transient receptor potential cation channel subfamily M.

Figures

Figure 1.
Figure 1.
Immunohistochemical staining of TRPM2 protein in bladder cancer and control samples. (A) Typical image of a normal tissue sample with high expression of TRPM2 protein. (B) Typical image of a bladder cancer sample with high expression of TRPM2 protein in bladder smooth muscle, and malignant cells invading the smooth muscle and the tunica media layer of blood vessels in a bladder cancer patient. TRPM2, transient receptor potential cation channel subfamily M member 2.
Figure 2.
Figure 2.
Fold changes in the mRNA levels of the TRPM genes. A comparison of the mRNA levels of TRPM2, TRPM4, TRPM5, TRPM7 and TRPM8 ion channel genes in bladder cancer patients and controls is shown. Gene expression levels were normalized according to the expression of glyceraldehyde 3-phosphate dehydrogenase. Results are presented as the mean ± standard deviation for all groups. ***P<0.05 vs. bladder control tissues. TRPM, transient receptor potential cation channel subfamily M.

Similar articles

See all similar articles

Cited by 3 articles

Feedback