Evaluation of sample extraction methods for minimizing hematocrit effect on whole blood analysis with volumetric absorptive microsampling

Bioanalysis. 2017 Feb;9(4):349-357. doi: 10.4155/bio-2015-0028. Epub 2017 Jan 19.

Abstract

Aim: Volumetric absorptive microsampler (VAMS) was designed to sample a fixed volume of blood regardless of the hematocrit (HCT) levels. Model compounds with a wide range of hydrophobicity were evaluated for their extraction recoveries from VAMS dried blood samples.

Results: For the highly hydrophobic compounds, recoveries with methanol or methanol/acetonitrile extraction were higher compared with using the aqueous mixture of methanol or acetonitrile. Extraction with methanol/acetonitrile (1:1) yielded more consistent recovery across the HCT range of 20-70% than using methanol alone, with good linearity, accuracy and precision achieved from 1 to 2000 ng/ml.

Conclusion: An organic solvent sample preparation approach was developed to optimize extraction recovery and minimize the HCT effect on the analysis of VAMS dried blood samples.

Keywords: hematocrit; microsampling; volumetric absorptive microsampler.

Publication types

  • Comparative Study

MeSH terms

  • Acetonitriles / chemistry
  • Blood Specimen Collection*
  • Chromatography, Liquid
  • Dried Blood Spot Testing / methods
  • Hematocrit*
  • Hematologic Tests
  • Humans
  • Linear Models
  • Methanol / chemistry
  • Reproducibility of Results
  • Solvents / chemistry
  • Specimen Handling / methods*
  • Tandem Mass Spectrometry*

Substances

  • Acetonitriles
  • Solvents
  • Methanol
  • acetonitrile