Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Jan 20;355(6322):280-284.
doi: 10.1126/science.aaj1562.

Root Diffusion Barrier Control by a Vasculature-Derived Peptide Binding to the SGN3 Receptor

Affiliations

Root Diffusion Barrier Control by a Vasculature-Derived Peptide Binding to the SGN3 Receptor

Verónica G Doblas et al. Science. .

Abstract

The root endodermis forms its extracellular diffusion barrier by developing ringlike impregnations called Casparian strips. A factor responsible for their establishment is the SCHENGEN3/GASSHO1 (SGN3/GSO1) receptor-like kinase. Its loss of function causes discontinuous Casparian strips. SGN3 also mediates endodermal overlignification of other Casparian strip mutants. Yet, without ligand, SGN3 function remained elusive. Here we report that schengen2 (sgn2) is defective in an enzyme sulfating peptide ligands. On the basis of this observation, we identified two stele-expressed peptides (CASPARIAN STRIP INTEGRITY FACTORS, CIF1/2) that complement sgn2 at nanomolar concentrations and induce Casparian strip mislocalization as well as overlignification-all of which depend on SGN3. Direct peptide binding to recombinant SGN3 identifies these peptides as SGN3 ligands. We speculate that CIF1/2-SGN3 is part of a barrier surveillance system, evolved to guarantee effective sealing of the supracellular Casparian strip network.

Comment in

Similar articles

See all similar articles

Cited by 35 articles

See all "Cited by" articles

Publication types

LinkOut - more resources

Feedback