Association of a missense mutation in the positional candidate gene glutamate receptor-interacting protein 1 with backfat thickness traits in pigs

Jae-Bong Lee; Hee-Bok Park; Chae-Kyoung Yoo; Hee-Sung Kim; In-Cheol Cho; Hyun-Tae Lim

doi:10.5713/ajas.16.0414

Association of a missense mutation in the positional candidate gene glutamate receptor-interacting protein 1 with backfat thickness traits in pigs

Asian-Australas J Anim Sci. 2017 Aug;30(8):1081-1085. doi: 10.5713/ajas.16.0414. Epub 2016 Dec 27.

Authors

Jae-Bong Lee¹, Hee-Bok Park², Chae-Kyoung Yoo³, Hee-Sung Kim⁴, In-Cheol Cho², Hyun-Tae Lim^{3

4}

Affiliations

¹ Korea Zoonosis Research Institute (KoZRI), Chonbuk National University, Iksan 54531, Korea.
² Subtropical Livestock Research Institute, National Institute of Animal Science, Jeju 63242, Korea.
³ Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Korea.
⁴ Division of Applied Life Science (BK21 plus), Gyeongsang National University, Jinju 52828, Korea.

Abstract

Objective: Previously, we reported quantitative trait loci (QTLs) affecting backfat thickness (BFT) traits on pig chromosome 5 (SW1482-SW963) in an F2 intercross population between Landrace and Korean native pigs. The aim of this study was to evaluate glutamate receptor-interacting protein 1 (GRIP1) as a positional candidate gene underlying the QTL affecting BFT traits.

Methods: Genotype and phenotype analyses were performed using the 1,105 F₂ progeny. A mixed-effect linear model was used to access association between these single nucleotide polymorphism (SNP) markers and the BFT traits in the F₂ intercross population.

Results: Highly significant associations of two informative SNPs (c.2442 T>C, c.3316 C>G [R1106G]) in GRIP1 with BFT traits were detected. In addition, the two SNPs were used to construct haplotypes that were also highly associated with the BFT traits.

Conclusion: The SNPs and haplotypes of the GRIP1 gene determined in this study can contribute to understand the genetic structure of BFT traits in pigs.

Keywords: Backfat; Glutamate Receptor-interacting Protein 1 (GRIP1); Korean Native Pigs; Landrace; Quantitative Trait Loci (QTL).