Real-Time Monitoring of Nanoparticle Formation by FRET Imaging

Angew Chem Int Ed Engl. 2017 Mar 6;56(11):2923-2926. doi: 10.1002/anie.201611288. Epub 2017 Jan 23.


Understanding the formation process of nanoparticles is of the utmost importance to improve their design and production. This especially holds true for self-assembled nanoparticles whose formation processes have been largely overlooked. Herein, we present a new technology that integrates a microfluidic-based nanoparticle synthesis method and Förster resonance energy transfer (FRET) microscopy imaging to visualize nanoparticle self-assembly in real time. Applied to different nanoparticle systems, for example, nanoemulsions, drug-loaded block-copolymer micelles, and nanocrystal-core reconstituted high-density lipoproteins, we have shown the approach's unique ability to investigate key parameters affecting nanoparticle formation.

Keywords: FRET; block co-polymers; high-density lipoproteins; microfluidics; nanoemulsions.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fluorescence Resonance Energy Transfer*
  • Nanoparticles / chemistry*
  • Time Factors